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    <Journal>
      <PublisherName>isfcppharmaspire</PublisherName>
      <JournalTitle>Pharmaspire</JournalTitle>
      <PISSN>C</PISSN>
      <EISSN>o</EISSN>
      <Volume-Issue>Volume 10, Issue 1</Volume-Issue>
      <PartNumber/>
      <IssueTopic>Multidisciplinary</IssueTopic>
      <IssueLanguage>English</IssueLanguage>
      <Season>January-March, 2018</Season>
      <SpecialIssue>N</SpecialIssue>
      <SupplementaryIssue>N</SupplementaryIssue>
      <IssueOA>Y</IssueOA>
      <PubDate>
        <Year>2022</Year>
        <Month>06</Month>
        <Day>14</Day>
      </PubDate>
      <ArticleType>Pharmaceutics</ArticleType>
      <ArticleTitle>Liquid chromatographic method for simultaneous estimation of Metformin HCl, Pioglitazone HCl and Glibenclamide in rat plasma</ArticleTitle>
      <SubTitle/>
      <ArticleLanguage>English</ArticleLanguage>
      <ArticleOA>Y</ArticleOA>
      <FirstPage>41</FirstPage>
      <LastPage>47</LastPage>
      <AuthorList>
        <Author>
          <FirstName>Gokul S. Talele</FirstName>
          <LastName/>
          <AuthorLanguage>English</AuthorLanguage>
          <Affiliation/>
          <CorrespondingAuthor>N</CorrespondingAuthor>
          <ORCID/>
          <FirstName>Durga Das Anghore</FirstName>
          <LastName/>
          <AuthorLanguage>English</AuthorLanguage>
          <Affiliation/>
          <CorrespondingAuthor>Y</CorrespondingAuthor>
          <ORCID/>
          <FirstName>Pawan K.</FirstName>
          <LastName>Porwal</LastName>
          <AuthorLanguage>English</AuthorLanguage>
          <Affiliation/>
          <CorrespondingAuthor>Y</CorrespondingAuthor>
          <ORCID/>
        </Author>
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      <DOI/>
      <Abstract>Separation of three analytes, namely metformin (MET), pioglitazone (PIO), and glibenclamide (GLB), was achieved within a single chromatographic run for the first time. Chromatographic method included stationary phase C18 Column (100 mm × 4.6 mm, 3.5 µm i.d.) and mobile phase consisting of ion-pair aqueous component and organic component in a gradient mode at 1 mL/min flow rate, and detection was monitored at 230 nm. Mobile-phase compositions and combinations were optimized for type and concentration of ion-pair reagents. Plasma sample preparation was based on protein precipitation by means of cold aqueous solution of 10% (w/v) trichloroacetic acid in combination with organic solvent addition. Ranitidine was used as the internal standard for MET, whereas rosiglitazone and amlodipine played the same role for PIO and GLB, respectively. Calibration curves were plotted from lower limit of quantification to 10,000 ng/mL for MET, PIO, and GLB. Weighing schemes of 1/X and 1/X2 were applied to observe goodness of fit in calibration curves. Precision was characterized by relative standard deviations below 15%. Stability analysis showed that all analytes are stable for at least 3 months when stored at ?20__degreesignC. The validated method was applied for determination of MET, PIO, and GLB in pharmacokinetic study samples.</Abstract>
      <AbstractLanguage>English</AbstractLanguage>
      <Keywords>Glibenclamide, high-performance liquid chromatography, metformin,  pharmacokinetic, pioglitazone, plasma</Keywords>
      <URLs>
        <Abstract>https://isfcppharmaspire.com/ubijournal-v1copy/journals/abstract.php?article_id=13848&amp;title=Liquid chromatographic method for simultaneous estimation of Metformin HCl, Pioglitazone HCl and Glibenclamide in rat plasma</Abstract>
      </URLs>
      <References>
        <ReferencesarticleTitle>References</ReferencesarticleTitle>
        <ReferencesfirstPage>16</ReferencesfirstPage>
        <ReferenceslastPage>19</ReferenceslastPage>
        <References>1. Aburuz S, Millership J, Elnay J. The development and validation of liquid chromatography method for the simultaneous determination of metformin and glipizide, gliclazide, glibenclamide or glimepiride in plasma. J Chromatogr B Anal Technol Biomed Life Sci 2005;817:277-86.&#13;
&#13;
2. Al-Rimawi F. Development and validation of an analytical method for metformin hydrochloride and its related compound (1-cyanoguanidine) in tablet formulations by HPLC-UV. Talanta 2009;79:1368-71.&#13;
&#13;
3. Amini H, Ahmadiani A, Gazerani P. Determination of metformin in rat plasma by high-performance liquid chromatography. J Chromatogr B Anal Technol Biomed Life Sci 2005;824:319-22.&#13;
&#13;
4. Block J, Beale JM. Wilson and Gisvold’s Organic Medicinal and Pharmaceutical Chemistry. 11th ed. Philadelphia, PA: Lippincott Williams and Wilkins; 2003. p. 956-60.&#13;
&#13;
5. Chaturvedi PK, Sharma R. Development and validation of an RP-HPLC method for simultaneous analysis of a three-component tablet formulation containing metformin hydrochloride, pioglitazone hydrochloride, and glibenclamide. Acta Chromatogr 2008;20:451-61.&#13;
&#13;
6. Chaturvedi PK, Sharma R. Simultaneous spectrophotometric estimation and validation of three-component tablet formulation containing pioglitazone hydrochloride, metformin hydrochloride and glibenclamide. Anal Lett 2008;41:2133-42.&#13;
&#13;
7. Gabr RQ, Padwal RS, Brocks DR. Determination of metformin in rat plasma and urine by high-performance liquid chromatography using small sample volume and conventional octadecyl silane column. J Pharm Pharm Sci 2010;13:486-94.&#13;
&#13;
8. Goswami L, Mukhoadhayay S, Durgapal S. Simultaneous estimation of metformin and pioglitazone by ultraviolet spectroscopy. Indian J Pharm Sci 2010;72:507-10.&#13;
&#13;
9. Granner DK, Davis SN. Endocrine pancreas and pharmacotherapy of diabetes mellitus and hypoglycaemia. In: Gilman AG, Hardman JG, Limbird LE, editors. Goodman and Gilman’s The Pharmacological Basis of Therapeutics. 12th ed. NewYork: McGraw-Hill; 2001. p. 1679-714.&#13;
&#13;
10. Jiang J, Feng F, Ma M, Zhang ZX. Study on a new precolumn derivatization method in the determination of metformin hydrochloride. J Chromatogr Sci 2006;44:193-9.&#13;
&#13;
11. Kahn CR, Weir GC, King GL, Jacobson AM, Moses AC, Smith RJ. Joslin’s Diabetes Mellitus. 14th ed. Boston: Lippincott Williams and Wilkins; 2007.&#13;
&#13;
12. Kar M, Chaudhary PK. HPLC method for estimation of metformin hydrochloride in formulated microspheres and tablet dosage form. Indian J Pharm Sci 2009;71:318-20.&#13;
&#13;
13. Koseki N, Kawashita H, Niina M, Nagae Y, Masuda N. Development and validation for high selective quantitative determination of metformin in rat plasma by cation exchanging with normal-phase LC/MS/MS. J Pharm Biomed Anal 2005;36:63-1072.&#13;
&#13;
14. Liu QF, Li ZD, Shi XJ, Jiao Z, Zhong MK. Simple and sensitive determination of metformin hydrochloride in plasma using an ion-pair LC method. Chromatographia 2009;70:1511-4.&#13;
&#13;
15. Snyder LR, Kirkland JJ, Glajch JL. Practical HPLC Method Development. 2nd ed. NewYork: John Wiley and Sons; 1997. p. 294-303.&#13;
&#13;
16. Sultana N, Arayne MS, Shafi N, Siddiqui FA, Hussain A. Development and validation of new assay method for the simultaneous analysis of diltiazem, metformin, pioglitazone and rosiglitazone by RP-HPLC and its applications in pharmaceuticals and rat serum. J Chromatogr Sci 2011;49:774-9.&#13;
&#13;
17. Tache F, Albu M. Specificity of an analytical HPLC assay method of metformin hydrochloride. Rev Roum Chim 2007;52:603-9.&#13;
&#13;
18. Tache F, David V, Farcab A, Medvedovicia A. HPLC-DAD determination of metformin in rat plasma using derivatization with p-nitrobenzoyl chloride in a biphasic system. Microb J 2001;68:13-9.&#13;
&#13;
19. Klaczkow G, Anuszewska EL. Determination of impurities in medical products containing metformin hydrochloride. Acta Pol Pharm Drug Res 2010;67:593-8.</References>
      </References>
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