<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.2d1 20170631//EN" "JATS-journalpublishing1.dtd">
<ArticleSet>
  <Article>
    <Journal>
      <PublisherName>isfcppharmaspire</PublisherName>
      <JournalTitle>Pharmaspire</JournalTitle>
      <PISSN>C</PISSN>
      <EISSN>o</EISSN>
      <Volume-Issue>Volume 10, Issue 1</Volume-Issue>
      <PartNumber/>
      <IssueTopic>Multidisciplinary</IssueTopic>
      <IssueLanguage>English</IssueLanguage>
      <Season>January-March, 2018</Season>
      <SpecialIssue>N</SpecialIssue>
      <SupplementaryIssue>N</SupplementaryIssue>
      <IssueOA>Y</IssueOA>
      <PubDate>
        <Year>2022</Year>
        <Month>06</Month>
        <Day>14</Day>
      </PubDate>
      <ArticleType>Pharmaceutics</ArticleType>
      <ArticleTitle>Simultaneous estimation of acebrophylline, montelukast, and levocetirizine dihydrochloride in marketed formulation by high-performance liquid chromatography method</ArticleTitle>
      <SubTitle/>
      <ArticleLanguage>English</ArticleLanguage>
      <ArticleOA>Y</ArticleOA>
      <FirstPage>23</FirstPage>
      <LastPage>28</LastPage>
      <AuthorList>
        <Author>
          <FirstName>Mohini</FirstName>
          <LastName>Mittal</LastName>
          <AuthorLanguage>English</AuthorLanguage>
          <Affiliation/>
          <CorrespondingAuthor>N</CorrespondingAuthor>
          <ORCID/>
          <FirstName>Yogesh</FirstName>
          <LastName>Upadhyay</LastName>
          <AuthorLanguage>English</AuthorLanguage>
          <Affiliation/>
          <CorrespondingAuthor>Y</CorrespondingAuthor>
          <ORCID/>
          <FirstName>Durgadas</FirstName>
          <LastName>Anghore</LastName>
          <AuthorLanguage>English</AuthorLanguage>
          <Affiliation/>
          <CorrespondingAuthor>Y</CorrespondingAuthor>
          <ORCID/>
          <FirstName>Anshul</FirstName>
          <LastName>Kumar</LastName>
          <AuthorLanguage>English</AuthorLanguage>
          <Affiliation/>
          <CorrespondingAuthor>Y</CorrespondingAuthor>
          <ORCID/>
          <FirstName>Ravindra K.</FirstName>
          <LastName>Rawal</LastName>
          <AuthorLanguage>English</AuthorLanguage>
          <Affiliation/>
          <CorrespondingAuthor>Y</CorrespondingAuthor>
          <ORCID/>
        </Author>
      </AuthorList>
      <DOI/>
      <Abstract>We have developed methods for simultaneous estimation of acebrophylline (ABP), montelukast (MLK), and levocetirizine dihydrochloride (levocetirizine [LCZ]) in pure and marketed formulation by reversed-phase high-performance liquid chromatography (HPLC). Method is simple, accurate, rapid, precise, economical, and reliable. The method was developed using waters HPLC an autosampler on column Macherey-Nagel C18 4.6 mm * 250 mm (5 µm) using a mixture of ammonium acetate buffer of pH 3.5 (pH adjusted with glacial acetic acid) and methanol in the ratio 15:85 v/v as mobile phase in an isocratic elution mode at a flow rate 0.6 ml/min, at 30__degreesignC with a load of 20 µl. Diluent used was water: methanol (50:50 v/v). The detection was carried out at 230 nm. Typical chromatogram with optimized condition gives sharp and symmetric peak with retention time of 5.287 min, 26.856 min, and 6.440 min for ABP, MLK, and LCZ, respectively. The method was validated with respect to linearity, robustness, precision, and accuracy and was successfully applied for the simultaneous quantitative determination of ABP, MLK, and LCZ in the solid dosage form and percentage purity were found to be 105%, 100%, and 96%, respectively.</Abstract>
      <AbstractLanguage>English</AbstractLanguage>
      <Keywords>Acebrophylline, HPLC, levocetirizine HCl, montelukast</Keywords>
      <URLs>
        <Abstract>https://isfcppharmaspire.com/ubijournal-v1copy/journals/abstract.php?article_id=13845&amp;title=Simultaneous estimation of acebrophylline, montelukast, and levocetirizine dihydrochloride in marketed formulation by high-performance liquid chromatography method</Abstract>
      </URLs>
      <References>
        <ReferencesarticleTitle>References</ReferencesarticleTitle>
        <ReferencesfirstPage>16</ReferencesfirstPage>
        <ReferenceslastPage>19</ReferenceslastPage>
        <References>1. Patel MM, Bhuva SD, Patel HD, Mori KN. An overview of the recent developments in analytical methodologies for determination of proton pump inhibitors in bulk drugs, pharmaceuticals and biological matrices. Eurasian J Anal Chem 2014;9:25-48.&#13;
&#13;
2. Prathap B. A review-importance of RP-HPLC in analytical method development. Int J Novel Trends Pharm Sci 2013;3:15-23.&#13;
&#13;
3. Ghebre-Sellassie I, Hem SL, Knevel AM. Separation of penicillin and its major degradation products by ion-pair reversed-phase high-pressure liquid chromatography. J Pharm Sci 1982;71:351-3.&#13;
&#13;
4. Maniya J, Raj H, Vaghani H, Mangukiya M, Dudhat P. Development and validation of spectroscopic method for simultaneous estimation of acebrophylline and montelukast sodium in combined dosage form. Indo Am J Pharm Res 2012;2:1027-36.&#13;
&#13;
5. Horvath C, Melander W. Liquid chromatography with hydrocarbonaceous bonded phases; theory and practice of reversed-phase chromatography. J Chromatogr Sci 1977;15:393-404.&#13;
&#13;
6. Vidyasagar G, Krs SR, Induri M, Ramanjeneyulu S. Development of validated liquid chromatographic method for estimation of levocetirizine from pharmaceutical dosage forms. J Appl Pharm Sci 2011;1:95.</References>
      </References>
    </Journal>
  </Article>
</ArticleSet>