PHARMASPIRE - Volume 10, Issue 3, July - September, 2018
Pages: 113-120
Date of Publication: 14-Jun-2022
Print Article
Download XML Download PDF
Effect of metformin and pioglitazone on serum albumin binding of selected sulfonylureas
Author: Navjot Kaur Sandhu, Sukhbir Kaur, Durga Das Anghore, Pawan Kumar Porwal
Category: Pharmaceutics
Abstract:
Metformin (MET) and pioglitazone (PIO) are known to induce conformation changes in the tertiary structure of human serum albumin and inhibit the non-enzymatic glycation of albumin. The pharmacological doses of sulfonylureas are about 100–500 time less as compared to MET and about 10–20 time less than PIO. The liquid chromatography coupled with tandem mass spectrometry was used to quantify all analytes. The retention of all analytes was made on a C8 column (50 mm × 2.1 mm and 2.5 µm) using 10 mM ammonium acetate buffer in gradient elution with acetonitrile at 0.25 ml/min flow rate and quantitated at selective reaction monitoring mode for the respective analyte. The Amicon® ultrafiltration device was used to access albumin binding studies. The analytes were spiked to 4% recombinant human serum albumin (rHSA) and 10% glycated human serum albumin (Gly-HSA) corresponding to their peak plasma concentration. Finally, the rHSA and Gly-HSA were incubated with various levels of MET, PIO, and sulfonylurea/s. The optimized bioanalytical liquid chromatography–tandem mass spectrometry was found linear in the range of 5–2000 ng/mL for MET and PIO, whereas, the calibration curve for glibenclamide and glimepiride was made in the range of 5–500 ng/mL. The lower limit of quantitation (LLOQ) for glibenclamide and glimepiride was 0.25 ng/mL, and LLOQ values for MET and PIO were 0.50 ng/mL and 1.0 ng/mL, respectively, with sufficient accuracy and precision. Competitive inhibition of non-enzymatic glycation of rHSA was observed in the presence of MET and PIO. An alteration of protein binding capacity was observed for sulfonylurea when incubated with glucose plus MET plus and PIO in comparison to control.
Keywords: Albumin, liquid chromatography–tandem mass spectrometry, metformin, pioglitazone, protein binding, sulfonylurea
References:
1. Bennett PH, Knowler WC. Definition, Diagnosis, and Classification of Diabetes Mellitus and Glucose Homeostasis. In: Kahn CR, Weir GC, King GL, Jacobson AM, Moses AC, Smith RJ, editors. Joslin’s Diabetes Mellitus. 14th ed. Massachusetts: Ovid Technologies, Inc.; 2006. p. 332-8.
2. Hohl CM, Dankoff J, Colacone A, Afilalo M. Polypharmacy, adverse drugrelated events, and potential adverse drug interactions in elderly patients presenting to an emergency department. Ann Emerg Med 2001;38:666-71.
3. Ibrahim IA, Kang E, Dansky KH. Polypharmacy and possible drug-drug interactions among diabetic patients receiving home health care services. Home Health Care Serv Q 2005;24:87-99.
4. Triplitt C. Drug interactions of medications commonly used in diabetes. Diabetes Spectrum 2006;19:202-11.
5. Nathan DM, Buse JB, Davidson MB, Ferrannini E, Holman RR, Sherwin R, et al. Medical management of hyperglycemia in type 2 diabetes: A consensus algorithm for the initiation and adjustment of therapy: A consensus statement of the American diabetes association and the European association for the study of diabetes. Diabetes Care 2009;32:193-203.
6. Bolen S, Feldman L, Vassy J, Wilson L, Yeh HC, Marinopoulos S, etal. Systematic review: Comparative effectiveness and safety of oral medications for Type 2 diabetes mellitus. Ann Intern Med 2007;147:386-99.
7. Duran G, Herschbach P, Waadt S, Strian F, Zettler A. Assessing daily problems with diabetes: A subject-oriented approach to compliance. Psychol Rep 1995;76:515-21.
8. Rees TD. Periodontal management of the patient with diabetes mellitus. Periodontol 2000 2000;23:63-72.
9. Benet LZ, Hoener BA. Changes in plasma protein binding have little clinical relevance. Clin Pharmacol Ther 2002;71:115-21.
10. Coussons PJ, Jacoby J, McKay A, Kelly SM, Price NC, Hunt JV, et al. Glucose modification of human serum albumin: A structural study. Free Radic Biol Med 1997;22:1217-27.
11. Shaklai N, Garlick RL, Bunn HF. Nonenzymatic glycosylation of human serum albumin alters its conformation and function. J Biol Chem 1984;259:3812-7.
12. Rondeau P, Navarra G, Cacciabaudo F, Leone M, Bourdon E, Militello V, et al. Thermal aggregation of glycated bovine serum albumin. Biochim Biophys Acta 2010;1804:789-98.
13. Oettl K, Stauber RE. Physiological and pathological changes in the redox state of human serum albumin critically influence its binding properties. Br J Pharmacol 2007;151:580-90.
14. Rahnama E, Mahmoodian-Moghaddam M, Khorsand-Ahmadi S, Saberi MR, Chamani J. Binding site identification of metformin to human serum albumin and glycated human serum albumin by spectroscopic and molecular modeling techniques: A comparison study. J Biomol Struct Dyn 2015;33:513-33.
15. Faure P, Wiernsperger N, Polge C, Favier A, Halimi S. Impairment of the antioxidant properties of serum albumin in patients with diabetes: Protective effects of metformin. Clin Sci 2008;114:251-6.
16. Sabín J, Prieto G, González-Pérez A, Ruso JM, Sarmiento F. Effects of fluorinated and hydrogenated surfactants on human serum albumin at different pHs. Biomacromolecules 2006;7:176-82.
17. Brée F, Urien S, Nguyen P, Tillement JP, Steiner A, Vallat-Molliet C, etal. Human serum albumin conformational changes as induced by tenoxicam and modified by simultaneous diazepam binding. J Pharm Pharmacol 1993;45:1050-3.
18. Khan MA, Muzammil S, Musarrat J. Differential binding of tetracyclines with serum albumin and induced structural alterations in drug-bound protein. Int J Biol Macromol 2002;30:243-9.
19. Kandagal PB, Ashoka S, Seetharamappa J, Shaikh SM, Jadegoud Y, Ijare OB, etal. Study of the interaction of an anticancer drug with human and bovine serum albumin: Spectroscopic approach. J Pharm Biomed Anal 2006;41:393-9.
20. Berezhkovskiy LM. On the influence of protein binding on pharmacological activity of drugs. J Pharm Sci 2010;99:2153-65.
21. Baraka-Vidot J, Guerin-Dubourg A, Bourdon E, Rondeau P. Impaired drug-binding capacities of in vitro and in vivo glycated albumin. Biochimie 2012;94:1960-7.
22. USFDA Draft Guideline for Industry on Bioanalytical Method Validation. U.S. Rockville, MD, USA: Department of Health and Human Services Food and Drug Administration (CDER & CVM); 2013.
23. Mereish KA, Rosenberg H, Cobby J. Glucosylated albumin and its influence on salicylate binding. J Pharm Sci 1982;71:235-8.
24. Porwal PK, Talele GS. Glycation alter serum albumin binding of valsartan and nateglinide when studied contemporarily. J Liq Chromatogr Relat Technol 2017;40:26-35.
25. Committee for Medicinal Products for Human Use. European Medicines Agency’s Guideline on Validation of Bioanalytical Methods. London: Committee for Medicinal Products for Human Use; 2012.
26. Beisswenger P. Metformin inhibition of glycation processes. Diabetes Metab 2003;29:95-103.
27. Miyata T, van Ypersele de Strihou C, Kurokawa K, Baynes JW. Alterations in nonenzymatic biochemistry in uremia: Origin and significance of ‘carbonyl stress’ in long-term uremic complications. Kidney Int 1999;55:389-99.
28. Miyata T, Ishikawa S, Asahi K, Inagi R, Suzuki D, Horie K, et al.2- isopropylidenehydrazono-4-oxo-thiazolidin-5-ylacetanilide (OPB-9195) treatment inhibits the development of intimal thickening after balloon injury of rat carotid artery: Role of glycoxidation and lipoxidation reactions in vascular tissue damage. FEBS Lett 1999;445:202-6.
29. Rahbar S, Natarajan R, Yerneni K, Scott S, Gonzales N, Nadler JL, et al. Evidence that pioglitazone, metformin and pentoxifylline are inhibitors of glycation. Clin Chim Acta 2000;301:65-77.
30. Iwashima Y, Eto M, Horiuchi S, Sano H. Advanced glycation end productinduced peroxisome proliferator-activated receptor gamma gene expression in the cultured mesangial cells. Biochem Biophys Res Commun 1999;264:441-8.
|