PHARMASPIRE - Volume 11, Issue 3, July - September, 2019

Pages: 76-78

Date of Publication: 14-Jun-2022

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In vitro anti-inflammatory and antioxidant potential of leaves of Canavalia gladiata

Author: Amarjit Kaur, Poonam Verma, Simranpreet Kaur, Vijender Kumar

Category: Pharmaceutics

Abstract:

Objective: The objective of this study was to evaluate the biological potential of different extracts of leaves of Canavalia gladiata by in vitro anti-inflammatory and antioxidant methods. Materials and Methods: An in vitro anti-inflammatory study was carried out by human red blood cell membrane stabilization method and antioxidant activity by 2, 2-diphenyl-1-picrylhydrazyl hydrate (DPPH) methods. Results: The in vitro membrane-stabilizing test performed on petroleum ether, chloroform, ethyl acetate, methanol, and aqueous extracts, at dose level 200 and 400 mg/kg and results showed dose-dependent activities and maximum activities founded at a higher dose as 51.89%, 58.99%, 68.12%, 67.34%, and 52.34% protection, respectively, as compared to control groups. The standard drug diclofenac (10 mg/kg), which showed 78.22% protection in membrane stabilization test and antioxidant potential at different doses levels 100, 200, and 400 mg/kg of extracts, found as 44.01%, 50.45%, 72.99%, 70.25%, and 78.10%, respectively, in DPPH free radical scavenging activity methods. The data’s statistically found significant as (a P < 0.01 and bP < 0.05) as compared to control. Conclusions: The present investigations have been confirmed the anti-inflammatory and antioxidant activities of leaves of C. gladiata that supports the traditional claims of C. gladiata as a medicinally active plant.

Keywords: Canavalia gladiata, anti-inflammatory, antioxidant, 2, 2-diphenyl-1- picrylhydrazyl hydrate methods, human red blood cell membrane stabilization

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