Pages: 145-151

Date of Publication: 10-Jun-2022

A comparative study of the in vitro antioxidant activity of dipeptidyl peptidase-4 inhibitors

Author: Partha Chattopadhyay, Manisha Singh, Abhijit De

Category: Pharmaceutics

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Abstract:

In vitro antioxidant activity assay is the preliminary step to determine a drug’s efficacy in combating oxidative stress when used in a clinical condition. Oxidative stress is a major concern in disease like diabetes mellitus where elevated level of glucose leads to higher generation of free radicals. The current study was aimed to determine the in vitro antioxidant activity of antidiabetic drug-the dipeptidyl peptidase-4 inhibitor (sitagliptin, vildagliptin, and saxagliptin) in combating the oxidative stress in diabetes mellitus. A total of five methods were adopted for determining the antioxidant potential of the drugs. The methods were 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, nitric oxide (NO), radical scavenging assay, phosphomolybdenum assay, assessment of inhibition of lipid peroxidation, and potassium ferricyanide mediated ferric reduction activity potential (PFRAP). The results indicated vildagliptin as best DPPH radical scavenger and saxagliptin as the best scavenger of NO radicals. Sitagliptin showed best reducing power and saxagliptin and sitagliptin showed promising results in inhibiting lipid peroxidation. These findings suggest that use of sitagliptin and saxagliptin in diabetic patient may control the hyperglycemic conditions well as other complications that are caused by hyperglycemia induced oxidative stress.

Keywords: 2, 2-diphenyl-1-picrylhydrazyl, FRAP, Lipid peroxidation, Phosphomolybdenum assay

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