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      <ArticleType>P'Ceutical Analysis</ArticleType>
      <ArticleTitle>Development Of a Validated Analytical Method for The Estimation of Sodium Fusidate in Pharmaceutical Dosage Form By RP-HPLC.</ArticleTitle>
      <Abstract>Introduction: The determination of sodium fusidate in pharmaceutical dose form was accomplished using a straightforward reverse phase HPLC approach. The separation via chromatography was accomplished by isocratic elution, on Waters e2695 HPLC system with Empower-3 software and 2998 module Photo Diode Array detectors configured with a pump that delivers quaternary solvents, an automated sample injector, and a column thermostat. A validated RP-HPLC method that is reproducible, rapid, simple, precise, rugged, economical and accurate has been developed.&#13;
Materials and Methods: Separation succeeded by using a Thermo Scientific Hypersil ODS (C18) Column (150 × 4.6 mm i.d. and 5 µm particle size), and methanol: 1% V/V glacial acetic acid: acetonitrile used as mobile phase in ratio of 10:30:60% v/v/v respectively. UV detector was used to detect the seperation at a wavelength of 235 nm with 1.1 ml/min of flow rate and volume of injection of 20 μl, was maintained during the elution with 10-minute run time.&#13;
Results: It was estimated that the retention time was 5.597 minutes with a correlation co-efficient of 0.999, the anticipated separation was found to be linear in the concentration range of 25-150 mcg/ml and it was found out that according to ICH regulations, every validation parameter fulfilled the acceptance criteria.&#13;
Conclusion: Validation of the chromatographic method was done for linearity, specificity, accuracy, precision, robustness, system suitability, Limit of detection (LOD) and limit of quantification (LOQ) study It can be utilised for sodium fusidate routine analysis in accordance with ICH recommendations.</Abstract>
      <Keywords>Sodium fusidate,RP- HPLC,Isocratic development,Method Validation</Keywords>
        <Abstract>https://isfcppharmaspire.com/ubijournal-v1copy/journals/abstract.php?article_id=14862&amp;title=Development Of a Validated Analytical Method for The Estimation of Sodium Fusidate in Pharmaceutical Dosage Form By RP-HPLC.</Abstract>