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      <Volume-Issue>Volume 13, Issue 4 </Volume-Issue>
      <Season>October - December, 2021</Season>
      <ArticleTitle>Formulation and development of botanicals-based herbal serum</ArticleTitle>
      <Abstract>Aim and Objective: Various botanicals are used in the management of various skin diseases such as Aloe vera, Green Tea, Wheatgrass, etc. Hence, in the treatment of skin diseases, various topical retinoids salicylic acid is used. Therefore, it is also found that botanicals such as green tea, A. vera, wheatgrass, etc have an anti-bacterial activity which helps in the management of skin problems. Materials and Methods: In this formulation, five batches are made with the change in concentrations of botanicals, i.e., A. vera and green tea. The botanicals which were used in the formulation were first checked for their phenolic content by high-performance thin-layer chromatography (HPTLC). In this, the standard of Gallic acid was taken and both the extracts samples were analyzed by HPTLC for the confirmation of the presence of gallic acid. The formulation was divided into two phases, i.e.,oil phase and aqueous phase. Both the phases are mixed properly Then, extract of A. vera and Green tea was added with continuous stirring. After that formulation was kept in a closed container for further study. Results: The result of the study revealed that the presence of Gallic acid in both the extracts was confirmed by HPTLC. Further, all the batches were evaluated for their pH, viscosity, and physical appearance and their results suggest that pH was 6.5, viscosity was 6580. On this basis, it was confirmed that batch F4 was a satisfactory formulation. In last it was concluded that F5 is a satisfactory herbal serum formulation. Conclusion: On this basis, it was confirmed that batch F4 was a satisfactory formulation. In last it was concluded that F5 is a satisfactory herbal serum formulation.</Abstract>
      <Keywords>Aloe vera, green tea, fingureprinting, herbal formulation, serum formulation</Keywords>
        <Abstract>https://isfcppharmaspire.com/ubijournal-v1copy/journals/abstract.php?article_id=13768&amp;title=Formulation and development of botanicals-based herbal serum</Abstract>
        <References>Cassano P, Petrie SR, Hamblin MR, Henderson TA, Iosifescu DV. Review of transcranial photobiomodulation for major depressive disorder: Targeting brain metabolism, inflammation, oxidative stress, and neurogenesis. Neurophotonics 2016;3:031404.&#13;
	Gollnick HJ. Current concepts of the pathogenesis of acne. Drugs 2003;63:1579-96.&#13;
	Adityan B, Kumari R, Thappa DM. Scoring systems in Acne vulgaris. Indian J Dermatol Venereol Leprol 2009;75:323-6.&#13;
	Fox L, Csongradi C, Aucamp M, Plessis JD, Gerber M. Treatment modalities for acne. Molecules 2016;21:1063.&#13;
	Latter G, Grice JE, Mohammed Y, Roberts MS, Benson HA. Targeted topical delivery of retinoids in the management of Acne vulgaris: Current formulations and novel delivery systems. Pharmaceutics 2019;11:490.&#13;
	Fourniand;egrave;re M, Latire T, Souak D, Feuilloley MG, Bedoux G. Staphylococcus epidermidis and Cutibacterium acnes: Two major sentinels of skin microbiota and the influence of cosmetics. Microorganisms 2020;8:1752.&#13;
	Maisch TJ. Anti-microbial photodynamic therapy: Useful in the future? Lasers Med Sci 2007;22:83-91.&#13;
	Decker A, Graber EM. Over-the-counter acne treatments: A review. J Clin Aesthet Dermatol 2012;5:32.&#13;
	Raghuwanshi K, Mishra A. Anti-acne phytoconstituents-an intensive review. Int J Rec Adv Med Pharma Res 2020;3:1.&#13;
	Simonart T. Newer approaches to the treatment of Acne vulgaris. Am J Clin Dermatol 2012;13:357-64.&#13;
	Thielitz A, Gollnick H. Topical retinoids in Acne vulgaris. Am J Clin Dermatol 2008;9:369-81.&#13;
	Dispenza MC, Wolpert EB, Gilliland KL, Dai JP, Cong Z, Nelson AM, et al. Systemic isotretinoin therapy normalizes exaggerated TLR-2-mediated innate immune responses in acne patients. J Invest Dermatol 2012;132:2198-205.&#13;
	Toyoda M, Morohashi MJ. An overview of topical antibiotics for acne treatment. Dermatology 1998;196:130-4.&#13;
	Elsaie ML. Hormonal treatment of Acne vulgaris: An update. Clin Cosmet Investig Dermatol 2016;9:241.&#13;
	Kim GK, Del Rosso JQ. Oral spironolactone in post-teenage female patients with Acne vulgaris: Practical considerations for the clinician based on current data and clinical experience. J Clin Aesthet Dermatol 2012;5:37.&#13;
	Barros B, Thiboutot D. Hormonal therapies for acne. Clin Dermatol 2017;35:168-72.&#13;
	Vance ME, Kuiken T, Vejerano EP, McGinnis SP, Hochella MF Jr., Rejeski D. Nanotechnology in the real world: Redeveloping the nanomaterial consumer products inventory. Beilstein J Nanotechnol 2015;6:1769-80.&#13;
	Tuominen A. Tannins and Other Polyphenols in Geranium Sylvaticum. UtuPub; 2018.&#13;
	Bezuidenhout JJ. Development of Vitamin C and E Fixed-dose Combination, Multiple-unit, Solid Oral Delivery Systems. Potchefstroom: North-West University, South-Africa; 2019.&#13;
	Pecquet C, Bayrou O, Vigan M, Raison N, Lauriere M. P14 hydrolysed wheat protein: A new allergen in cosmetics and food. Contact Dermat 2004;50:182-3.&#13;
	Adams RM, Maibach HI. A five-year study of cosmetic reactions. J Am Acad Dermatol 1985;13:1062-9.&#13;
	Burlando B, Verotta L, Cornara L, Bottini-Massa E. Herbal Principles in Cosmetics: Properties and Mechanisms of Action. United States: CRC Press; 2010.&#13;
	Salvioni L, Morelli L, Ochoa E, Labra M, Fiandra L, Palugan L, et al. The emerging role of nanotechnology in skincare. Adv Coll Interface Sci 2021;293:102437.&#13;
	Sorg O, Antille C, Kaya G, Saurat JH. Retinoids in cosmeceuticals. Dermatol Ther 2006;19:289-96.&#13;
	Kedishvili NY. Retinoic acid synthesis and degradation. Subcell Biochem 2016;81:127-61.&#13;
	Zasada M, Budzisz E. Retinoids: Active molecules influencing skin structure formation in cosmetic and dermatological treatments. Postepy Dermatol Alergol 2019;36:392.&#13;
	Saniasiaya DJ. Antifungal effect of Malaysian Aloe vera leaf extract on selected fungal species of pathogenic otomycosis in in vitro culture medium. Oman Med J 2017;32:41.&#13;
	Howlader MM, Ahmed SR, Kubra K, Bhuiyan KH. Biochemical and phytochemical evaluation of Stevia rebaudiana. Asian J Med Biol Res 2016;2: 121-30.&#13;
	Karthik R. Pharmacognostical, Phytochemical Pharmacological Evaluation of Justicia Glauca. Madurai: College of Pharmacy, Madurai Medical College; 2012.&#13;
	Pandey DK, Shahnawaz, Dey A. Comparative HPTLC analysis of antioxidant compound gallic acid from in vitro and naturally grown Stevia rebaudiana. J Biol Active Prod Nat 2015;5:397-405.&#13;
	Pandey DK, Parida S, Dey A. Comparative HPTLC analysis of bioactive marker barbaloin from in vitro and naturally grown Aloe vera. Rev Bras Farmacogn 2016;26:161-7.&#13;
	Nawarathne NW, Wijesekera K, Wijayaratne WM, Napagoda M. Development of novel topical cosmeceutical formulations from Nigella sativa L. with antimicrobial activity against acne-causing microorganisms. ScientificWorldJournal 2019;2019:5985207.</References>