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[An Official Publication of ISF College of Pharmacy, Moga]


 

Original Article
Year : 2018   |  Volume : 10  |  Issue : 1  |  Page : 23-28

Simultaneous estimation of acebrophylline, montelukast, and levocetirizine dihydrochloride in marketed formulation by high-performance liquid chromatography method

We have developed methods for simultaneous estimation of acebrophylline (ABP), montelukast (MLK), and levocetirizine dihydrochloride (levocetirizine [LCZ]) in pure and marketed formulation by reversed-phase high-performance liquid chromatography (HPLC). Method is simple, accurate, rapid, precise, economical, and reliable. The method was developed using waters HPLC an autosampler on column Macherey-Nagel C18 4.6 mm * 250 mm (5 μm) using a mixture of ammonium acetate buffer of pH 3.5 (pH adjusted with glacial acetic acid) and methanol in the ratio 15:85 v/v as mobile phase in an isocratic elution mode at a flow rate 0.6 ml/min, at 30°C with a load of 20 μl. Diluent used was water:methanol (50:50 v/v). The detection was carried out at 230 nm. Typical chromatogram with optimized condition gives sharp and symmetric peak with retention time of 5.287 min, 26.856 min, and 6.440 min for ABP, MLK, and LCZ, respectively. The method was validated with respect to linearity, robustness, precision, and accuracy and was successfully applied for the simultaneous quantitative determination of ABP, MLK, and LCZ in the solid dosage form and percentage purity were found to be 105%, 100%, and 96%, respectively.
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